ABSTRACT
Rhinosporidiosis is primarily an infection of the nose caused by Rhinosporidium seeberi. Other sites that can be involved are conjunctiva, urethra, palate, tongue, epiglottis, larynx, trachea, bronchi, skin, vulva, and vagina. It is endemic in certain parts of India and Sri Lanka. The pathogen is difficult to grow in culture and Hematoxylin and Eosin staining helps in confirmation of the etiological agent. Surgery remains the mainstay of treatment. Here is a report aimed at documenting a 26-year-old female patient with nasal rhinosporidiosis.
ABSTRACT
The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66 percent, 86.66 percent and 93.33 percent respectively and the specificity of the assay was 70 percent for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Results of the present study show that cyst wall and protoscolex can also be an useful source of antigen in detection of hydatid antibodies in the serodiagnosis of CE.
O propósito do presente trabalho é avaliar a parede cística e protoscolex como fontes alternativas de antígeno no sorodiagnóstico de equinococose cística (CE). De um total de 90 amostras de sangue, foram coletadas 30 de casos CE confirmados, 30 de controles de doença e 30 controles saudáveis. Dot-Elisa usando parede cística, protoscolex e fluido cístico foi utilizada para demonstrar anticorpos anti-hidáticos. A sensitividade de Dot-Elisa usando parede cística, protoscolex e fluido cístico foi de: 96,66 por cento, 86,66 por cento e 93,33 por cento respectivamente e a especificidade do ensaio de 70 por cento para Dot-Elisa usando fluido cístico, protoscolex e antígeno da parede cística. Resultados do presente estudo mostram que parede cística e protoscolex podem ser fontes úteis de antígeno na detecção de anticorpos hidáticos no sorodiagnóstico do CE.
Subject(s)
Animals , Humans , Antibodies, Helminth/blood , Antigens, Helminth , Echinococcosis, Hepatic/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Immunoglobulin G/blood , Antigens, Helminth/immunology , Case-Control Studies , Echinococcosis, Hepatic/immunology , Predictive Value of Tests , Sensitivity and SpecificityABSTRACT
A short term investigation on the Campylobacter enteritis among children under 10 years of age was carried out in Chennai. The study revealed an isolation rate of 11 per cent in 100 patients suffering from acute diarrhoea comprising C. jejuni (8%) and C. coli. (3%). Among the two culture methods used, the candle jar method was found to be superior to plastic bag incubation system in recovering campylobacters on charcoal cefeperazone deoxycholate agar. While all the isolates were sensitive to ciprofloxacin, all of them exhibited resistance to nalidixic acid.